Start learning →

AP Intermediate 2nd Year Biotechnology and Applications — practice questions

Explore 167 free Biotechnology and Applications questions in AP Intermediate 2nd Year Biology. Open each question or exercise to read its content, reference answers and available explanation.

Practise Biotechnology and Applications with Quill →

Biotechnology and Applications questions and exercises with answers

Showing questions 1–30 of 167.

Restriction enzymes are best described as:Read the answer and worked solution →In recombinant DNA technology, plasmids are commonly used as:Read the answer and worked solution →The three sequential steps of one PCR cycle are:Read the answer and worked solution →Taq DNA polymerase used in PCR is obtained from:Read the answer and worked solution →In gel electrophoresis, DNA fragments move toward the:Read the answer and worked solution →The standard PLANT cell transformation method uses:Read the answer and worked solution →The first MAJOR recombinant DNA pharmaceutical, released commercially in 1982, was:Read the answer and worked solution →Bt cotton is resistant to BOLLWORM pests because it carries a gene from:Read the answer and worked solution →Match each biotechnology term to its meaning.Read the answer and worked solution →Match each biotechnology term to its meaning.Read the answer and worked solution →NEET · Biology · Biotech — Medical or AgriculturalRead the answer and worked solution →Run one PCR cycle in orderRead the answer and worked solution →Which enzyme joins two cut DNA ends to make recombinant DNA?Read the answer and worked solution →The first restriction endonuclease to be isolated was:Read the answer and worked solution →Hind II cuts DNA on recognising a specific sequence of:Read the answer and worked solution →Restriction enzymes are popularly called:Read the answer and worked solution →Nucleases that remove nucleotides from DNA ends are:Read the answer and worked solution →In gel electrophoresis DNA fragments move towards the:Read the answer and worked solution →Which matrix is most commonly used to separate DNA fragments?Read the answer and worked solution →In an agarose gel the fragment that travels farthest is the:Read the answer and worked solution →Separated DNA bands become visible after staining with:Read the answer and worked solution →Cutting a stained DNA band out of the gel and recovering it is:Read the answer and worked solution →The vector sequence from which replication begins is the:Read the answer and worked solution →An antibiotic resistance gene on a vector serves mainly as a:Read the answer and worked solution →A foreign gene ligated at the BamH I site of pBR322 destroys:Read the answer and worked solution →Colonies whose beta galactosidase gene carries an insert appear:Read the answer and worked solution →Loss of gene function caused by a DNA insert inside it is:Read the answer and worked solution →Which plasmid was reworked into a cloning vector for plants?Read the answer and worked solution →Bacterial cells are made competent by treating them with a divalent:Read the answer and worked solution →Recombinant DNA uptake is helped by a brief heat shock at:Read the answer and worked solution →

Worked practice questions

Restriction enzymes are best described as:

  1. Enzymes that join DNA fragments together
  2. Enzymes that cut DNA at specific sequences
  3. Enzymes that replicate DNA in the cell
  4. Enzymes that unwind DNA helices
Show answer and solution

Correct answer: B. Enzymes that cut DNA at specific sequences

Restriction endonucleases cut DNA at specific sequences (restriction sites, often 4-8 bp palindromes). Joining is done by DNA ligase. Replication by DNA polymerase. Unwinding by helicase.

Open this question →

In recombinant DNA technology, plasmids are commonly used as:

  1. Restriction enzymes for cutting DNA
  2. Selection markers for antibiotic resistance
  3. Vectors for carrying foreign genes into hosts
  4. Primers used in PCR amplification
Show answer and solution

Correct answer: C. Vectors for carrying foreign genes into hosts

Plasmids are small circular extra-chromosomal DNA molecules in bacteria. They are commonly used as VECTORS to carry foreign genes into host cells (typically E. coli). Plasmid pBR322 is a textbook example.

Open this question →

The three sequential steps of one PCR cycle are:

  1. Mixing, heating, cooling repeatedly
  2. Cutting, joining, amplifying again
  3. Replication, transcription, translation
  4. Denaturation, annealing, extension
Show answer and solution

Correct answer: D. Denaturation, annealing, extension

**One PCR cycle:** (1) DENATURATION at 94°C (DNA strands separate) (2) ANNEALING at 50-60°C (primers bind) (3) EXTENSION at 72°C (Taq polymerase extends). 30 cycles ≈ 10⁹ fold amplification.

Open this question →

How to practise

  1. Choose a Biotechnology and Applications question and try to work out your answer.
  2. Open the question to check the correct answer and read its worked solution.
  3. Continue in the app for bite-sized lessons, explanations and review with Quill. You can start without creating an account.

Put it into practice

Continue with Biotechnology and Applications practice for AP Intermediate 2nd Year. Read explanations as you go. No signup form needed to start.

Practise Biotechnology and Applications →

More Biology topics

Genetics and EvolutionReproduction in Organisms & PlantsBiology in Human Welfare & HealthEcology and EnvironmentReproductive HealthOrganisms and PopulationsBiodiversity and ConservationEvolution