A foreign gene ligated at the BamH I site of pBR322 destroys:
AErythromycin resistance
BSulphonamide resistance
CTetracycline resistance
DStreptomycin resistance
Answer & Solution
Correct answer: C. Tetracycline resistance
1. That BamH I site lies inside the tetracycline resistance gene.
2. An insert there interrupts the gene.
3. The recombinants therefore lose tetracycline resistance.
_Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_
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