Home › UP Board Class 12 › biology › Biotechnology: Principles and Processes › The three sequential steps of one PCR cycle are:
The three sequential steps of one PCR cycle are:
AMixing, heating, cooling repeatedly
BCutting, joining, amplifying again
CReplication, transcription, translation
DDenaturation, annealing, extension
Answer & Solution
Correct answer: D. Denaturation, annealing, extension
One PCR cycle: (1) DENATURATION at 94°C (DNA strands separate); (2) ANNEALING at 50-60°C (primers bind); (3) EXTENSION at 72°C (Taq polymerase extends). 30 cycles ≈ 10⁹ fold amplification.
Related questions
Making multiple identical copies of a specific piece of DNA by linking it to an ori is terBiotechnology, per the European Federation of Biotechnology (EFB), is best defined asA "vector" in recombinant DNA technology serves toThe first restriction endonuclease that recognised a specific 6-base sequence and cut DNA Which of the following is NOT a step in genetically modifying an organism as per NCERT?A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting ofNucleases fall into two broad categories:The presence of an "origin of replication" is important because it