alleryThe three sequential steps of one PCR cycle are:
AMixing, heating, cooling repeatedly
BCutting, joining, amplifying again
CReplication, transcription, translation
DDenaturation, annealing, extension
Answer & Solution
Correct answer: D. Denaturation, annealing, extension
**One PCR cycle:** (1) DENATURATION at 94°C (DNA strands separate)
(2) ANNEALING at 50-60°C (primers bind)
(3) EXTENSION at 72°C (Taq polymerase extends).
30 cycles ≈ 10⁹ fold amplification.
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