alleryThe standard PLANT cell transformation method uses:
ACaCl2 + heat shock (bacterial method)
BElectroporation (mainly animal cells)
CAgrobacterium tumefaciens with Ti plasmid
DMicroinjection with a glass needle
Answer & Solution
Correct answer: C. Agrobacterium tumefaciens with Ti plasmid
Agrobacterium tumefaciens is a natural plant pathogen
its Ti plasmid transfers DNA into plant cells.
A DISARMED (tumour-disabled) version is the standard plant biotech vector.
CaCl2-heat is for E. coli.
Electroporation + microinjection for animal cells.
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