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Bacterial cells are made competent by treating them with a divalent:

ANitrate ion
BAcetate ion
CCalcium ion
DChloride ion
Answer & Solution
Correct answer: C. Calcium ion
1. DNA is hydrophilic and cannot cross a membrane. 2. A divalent cation opens pores in the cell wall. 3. Calcium is the cation used for this. _Source: NCERT Class 12 Biology, Ch 9 'Biotechnology : Principles and Processes'_
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