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AP Intermediate 2nd Year Biotechnology and Applications — practice questions — page 2

Explore 167 free Biotechnology and Applications questions in AP Intermediate 2nd Year Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 31–60 of 167.

Bombarding plant cells with DNA coated gold particles is called:Read the answer and worked solution →Micro-injection puts recombinant DNA directly into the:Read the answer and worked solution →PCR amplifies a chosen segment using two sets of:Read the answer and worked solution →The thermostable polymerase used in PCR is taken from:Read the answer and worked solution →Repeated cycles of PCR can amplify a segment about:Read the answer and worked solution →Which enzyme is used to open plant cells before DNA isolation?Read the answer and worked solution →Purified DNA finally precipitates out on adding chilled:Read the answer and worked solution →Separation and purification of the final product is called:Read the answer and worked solution →Match each tool to the job it doesRead the answer and worked solution →Order the steps of gene cloningRead the answer and worked solution →True or false about restriction enzymesRead the answer and worked solution →Sort each item into the right boxRead the answer and worked solution →Fill the gaps in the cloning storyRead the answer and worked solution →Spot the one that does not fitRead the answer and worked solution →Which engineering does each job belong to?Read the answer and worked solution →Pick the right answer, quicklyRead the answer and worked solution →The power of any plant cell to grow into a whole plant is:Read the answer and worked solution →Raising thousands of plants through tissue culture is called:Read the answer and worked solution →Plants raised from one parent by tissue culture are called:Read the answer and worked solution →Virus free plants can be raised from an infected plant using its:Read the answer and worked solution →Plant cells stripped of their walls by digestion are called:Read the answer and worked solution →Fusing protoplasts of two varieties gives a plant known as a:Read the answer and worked solution →Rice enriched with vitamin A by genetic modification is:Read the answer and worked solution →The Bt toxin used in pest resistant crops comes from a:Read the answer and worked solution →Inside Bacillus thuringiensis the Bt toxin is stored as:Read the answer and worked solution →The Bt protoxin becomes an active toxin because the insect gut is:Read the answer and worked solution →Once activated the Bt toxin kills by acting on the insect:Read the answer and worked solution →The genes cryIAc and cryIIAb protect cotton against:Read the answer and worked solution →Tobacco roots are protected from a nematode by silencing its:Read the answer and worked solution →Silencing a gene with a complementary double stranded RNA is:Read the answer and worked solution →

Worked practice questions

Bombarding plant cells with DNA coated gold particles is called:

  1. Biolistics
  2. Sonication
  3. Elutriation
  4. Hybridising
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Correct answer: A. Biolistics

1. High velocity micro particles of gold or tungsten carry the DNA. 2. They are fired straight through the cell wall. 3. This gene gun method is called biolistics. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Micro-injection puts recombinant DNA directly into the:

  1. Cytosol
  2. Nucleus
  3. Ribosome
  4. Lysosome
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Correct answer: B. Nucleus

1. The needle bypasses the cell membrane entirely. 2. It is aimed at the animal cell nucleus. 3. The DNA is delivered there directly. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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PCR amplifies a chosen segment using two sets of:

  1. Vectors
  2. Plasmid
  3. Primers
  4. Ligases
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Correct answer: C. Primers

1. The polymerase cannot start on bare template. 2. Short synthetic oligonucleotides mark where to begin. 3. These are the two sets of primers. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Genetics and EvolutionReproduction in Organisms & PlantsBiology in Human Welfare & HealthEcology and EnvironmentReproductive HealthOrganisms and PopulationsBiodiversity and ConservationEvolution