CBSE Class 12 (Science) Biotechnology: Principles and Processes — practice questions
95 free MCQs with worked solutions. Tap any question for the answer + explanation, or practice them all in the app.
Practice CBSE Class 12 (Science) Biotechnology: Principles and Processes in the app →Restriction enzymes are best described as:In recombinant DNA technology, plasmids are commonly used as:The three sequential steps of one PCR cycle are:Taq DNA polymerase used in PCR is obtained from:In gel electrophoresis, DNA fragments move toward the:The standard PLANT cell transformation method uses:The first MAJOR recombinant DNA pharmaceutical, released commercially in 1982, was:Bt cotton is resistant to BOLLWORM pests because it carries a gene from:Which enzyme joins two cut DNA ends to make recombinant DNA?The first restriction endonuclease to be isolated was:Hind II cuts DNA on recognising a specific sequence of:Restriction enzymes are popularly called:Nucleases that remove nucleotides from DNA ends are:In gel electrophoresis DNA fragments move towards the:Which matrix is most commonly used to separate DNA fragments?In an agarose gel the fragment that travels farthest is the:Separated DNA bands become visible after staining with:Cutting a stained DNA band out of the gel and recovering it is:The vector sequence from which replication begins is the:An antibiotic resistance gene on a vector serves mainly as a:A foreign gene ligated at the BamH I site of pBR322 destroys:Colonies whose beta galactosidase gene carries an insert appear:Loss of gene function caused by a DNA insert inside it is:Which plasmid was reworked into a cloning vector for plants?Bacterial cells are made competent by treating them with a divalent:Recombinant DNA uptake is helped by a brief heat shock at:Bombarding plant cells with DNA coated gold particles is called:Micro-injection puts recombinant DNA directly into the:PCR amplifies a chosen segment using two sets of:The thermostable polymerase used in PCR is taken from:Repeated cycles of PCR can amplify a segment about:Which enzyme is used to open plant cells before DNA isolation?Purified DNA finally precipitates out on adding chilled:Separation and purification of the final product is called:In which year did Cohen and Boyer construct the first recombinant DNA?The first recombinant DNA used a plasmid of which bacterium?Which enzyme joins the cut ends of a gene and a plasmid vector?Molecular scissors used to cut DNA at specific sites are called what?Which technique maintains sterile conditions for growing only the desired cells at large scale?Altering the chemistry of DNA and RNA to change a host's phenotype is called what?Which specific DNA sequence initiates replication of a chromosome?Why must alien DNA be linked to an origin of replication in a host?A recombinant plasmid placed in E. coli replicates using whose DNA polymerase?How many basic steps are listed for genetically modifying an organism?Which of these is NOT a key tool of recombinant DNA technology?Which was the first restriction endonuclease whose action depended on a specific sequence?Hind II recognises a specific sequence of how many base pairs?In the name EcoRI, what does the first letter E stand for?In EcoRI, the letter R is derived from what?Roman numerals in a restriction enzyme name such as EcoRI indicate what?Which enzymes remove nucleotides only from the ends of DNA?Which nucleases make cuts at specific positions inside the DNA?Restriction endonucleases recognise which type of nucleotide sequence?How does a palindromic DNA sequence read the same?The sequence recognised by EcoRI on one strand is which of these?Where do restriction enzymes cut a palindromic site?What are the overhanging single-stranded ends left after cutting called?Sticky ends join to complementary ends by forming which bonds?Why cut vector and source DNA with the same enzyme?Which technique separates DNA fragments according to their size?The most commonly used matrix for DNA electrophoresis is made from what?DNA bands in a gel are made visible by staining with which compound?Stained DNA bands in a gel glow under which radiation?What is the step of extracting DNA bands cut from the gel called?Which two natural elements serve as bacterial cloning vectors?Some plasmids have how many copies per cell?Which vector feature controls the copy number of linked DNA?What is the function of a selectable marker in a vector?Which gene is a useful selectable marker for E. coli?Which E. coli cloning vector carries ampR and tetR genes?Foreign DNA inserted at the BamH I site of pBR322 inactivates which gene?Recombinant pBR322 colonies show which pattern on antibiotic plates?Which enzyme's gene is used for blue-white colony screening of recombinants?With a chromogenic substrate, non-recombinant colonies appear what colour?The loss of a gene's function due to an insert within it is called what?Which vectors are disarmed and used to deliver genes into animal cells?Why must bacterial cells be made competent to take up DNA?Which divalent cation is used to make bacteria competent?The heat shock step for bacterial transformation is given at what temperature?Direct injection of recombinant DNA into an animal cell nucleus is called what?The gene gun method uses particles of which metals coated with DNA?Which enzyme breaks open bacterial cells to release DNA?Which enzyme removes RNA during DNA isolation?The primers used in PCR are what kind of molecules?Which bacterium yields the thermostable DNA polymerase used in PCR?What are the three steps of each PCR cycle in order?If recombinant DNA with an ampicillin resistance gene enters E. coli, what happens?A protein from a gene expressed in a heterologous host is called what?In continuous culture, cells are kept in which phase of growth?What is a bioreactor?Bioreactors typically process how much culture volume?The most commonly used bioreactors are of which type?In a sparged stirred-tank bioreactor, what is bubbled through the culture?Separation and purification of a product after biosynthesis are collectively called what?The EFB defines biotechnology as the integration of what?