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NEET UG Biotechnology: Principles and Processes — practice questions — page 2

Explore 84 free Biotechnology: Principles and Processes questions in NEET UG Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology: Principles and Processes questions and exercises with answers

Showing questions 31–60 of 84.

Towards which electrode do DNA fragments move in a gel?Read the answer and worked solution →Agarose, the common gel matrix for DNA, is extracted from what?Read the answer and worked solution →Which compound is used to stain DNA so its bands show under UV light?Read the answer and worked solution →What colour do ethidium bromide stained DNA bands show under UV light?Read the answer and worked solution →Cutting separated DNA bands out of agarose and extracting them is called what?Read the answer and worked solution →Besides starting replication, ori also controls what?Read the answer and worked solution →What does a selectable marker in a vector do?Read the answer and worked solution →The procedure that introduces a piece of DNA into a host bacterium is called what?Read the answer and worked solution →Why should a vector carry a single site for a given restriction enzyme?Read the answer and worked solution →Foreign DNA ligated at the BamH I site of pBR322 makes the plasmid lose which trait?Read the answer and worked solution →After insertion at the BamH I site of pBR322, recombinants grow how?Read the answer and worked solution →What is it called when an insert disrupts a vector gene and stops its product?Read the answer and worked solution →Colour-based selection of recombinants inserts DNA into the gene of which enzyme?Read the answer and worked solution →With a chromogenic substrate, colonies whose plasmid carries an insert appear how?Read the answer and worked solution →Why is antibiotic-inactivation selection called cumbersome?Read the answer and worked solution →Which dicot pathogen delivers T-DNA to cause plant tumours?Read the answer and worked solution →In the Ti plasmid of Agrobacterium, what does Ti stand for?Read the answer and worked solution →Which disarmed agents are used as vectors to deliver genes into animal cells?Read the answer and worked solution →Why can DNA not pass through cell membranes on its own?Read the answer and worked solution →Treating bacteria with ions of which element makes them competent to take up DNA?Read the answer and worked solution →At what temperature is the brief heat shock given to competent E. coli cells?Read the answer and worked solution →Injecting recombinant DNA straight into an animal cell nucleus is called what?Read the answer and worked solution →In the gene gun method, DNA is coated onto micro-particles of which metals?Read the answer and worked solution →Which enzyme is used to break open fungal cells to release their DNA?Read the answer and worked solution →During DNA isolation, contaminating RNA is removed by treatment with what?Read the answer and worked solution →Purified DNA precipitates out as fine threads after adding which reagent?Read the answer and worked solution →The threads of DNA that separate out after precipitation are removed by what?Read the answer and worked solution →Which technique is used to check how far a restriction digestion has progressed?Read the answer and worked solution →What does the abbreviation PCR stand for?Read the answer and worked solution →In PCR, what are primers?Read the answer and worked solution →

Worked practice questions

Towards which electrode do DNA fragments move in a gel?

  1. Anode, the positive electrode
  2. Cathode, the negative electrode
  3. Neither, they stay in the wells
  4. Both electrodes equally
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Correct answer: A. Anode, the positive electrode

DNA carries a negative charge, so it migrates to the positive electrode, the anode.

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Agarose, the common gel matrix for DNA, is extracted from what?

  1. Cotton fibres
  2. Animal bones
  3. Seaweeds
  4. Fungal walls
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Correct answer: C. Seaweeds

Agarose is a natural polymer obtained from seaweeds.

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Which compound is used to stain DNA so its bands show under UV light?

  1. Methylene blue
  2. Ethidium bromide
  3. Crystal violet
  4. Aceto-carmine
Show answer and solution

Correct answer: B. Ethidium bromide

Pure DNA is invisible, so it is stained with ethidium bromide and viewed under UV radiation.

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