alleryAt what temperature is the brief heat shock given to competent E. coli cells?
A37 degrees Celsius
B42 degrees Celsius
C65 degrees Celsius
D94 degrees Celsius
Answer & Solution
Correct answer: B. 42 degrees Celsius
Cells on ice with recombinant DNA are briefly moved to 42 degrees Celsius and returned to ice so they take up the DNA.
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