alleryAfter insertion at the BamH I site of pBR322, recombinants grow how?
AOn both ampicillin and tetracycline
BOn tetracycline but not ampicillin
COn ampicillin but not tetracycline
DOn neither of the two antibiotics
Answer & Solution
Correct answer: C. On ampicillin but not tetracycline
The insert disrupts only the tetracycline gene, so recombinants keep ampicillin resistance but die on tetracycline.
Keep practising Biotechnology: Principles and Processes
Browse all Biotechnology: Principles and Processes questions for Bihar Board Class 12 (Science), or explore more Biology topics.
Related questions
In which year did Cohen and Boyer construct the first recombinant DNA?Why is a buffer used during gel electrophoresis?Typical type II restriction enzymes usually need which cofactor alone?On average, a random 6-base recognition site occurs once in about how many base pairs?Enzymes that recognise the same site but cut at a different position are called what?What natural role do restriction enzymes play inside bacteria?Separation and purification of a product after biosynthesis are together called what?Which type of bioreactor is the most commonly used?