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A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting of

A3' overhang of AATT
B5' overhang of AATT
C5' overhang of GAAT
DBlunt ends only
Answer & Solution
Correct answer: B. 5' overhang of AATT
1. EcoRI recognises 5'-GAATTC-3' and cuts between G and A on the top strand. 2. On the complementary strand it cuts between G and A (mirror image). 3. This produces single-stranded 5' overhangs of 5'-AATT-3'. 4. The overhangs are the sticky ends that pair with complementary AATT overhangs. _Source: syllabus Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2.1_
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