A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting…

Question

A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting of

Accepted Answer

B. {'text': "5' overhang of AATT", 'label': 'B'}

1. EcoRI recognises 5'-GAATTC-3' and cuts between G and A on the top strand.
2. On the complementary strand it cuts between G and A (mirror image).
3. This produces single-stranded 5' overhangs of 5'-AATT-3'.
4. The overhangs are the sticky ends that pair with complementary AATT overhangs.
_Source: NCERT Class 12 Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2.1_

Answer

{'text': "3' overhang of AATT", 'label': 'A'}

Answer

{'text': "5' overhang of GAAT", 'label': 'C'}

Answer

{'text': 'Blunt ends only', 'label': 'D'}