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The role of DNA ligase in recombinant DNA technology is to

ACut DNA at palindromic sites
BJoin the ends of two DNA fragments
CCopy DNA into RNA
DDigest single-stranded DNA
Answer & Solution
Correct answer: B. Join the ends of two DNA fragments
1. Restriction enzymes cut DNA; DNA ligase joins DNA. 2. Ligase forms a phosphodiester bond between adjacent nucleotides on a cut DNA strand. 3. It seals the sugar-phosphate backbone of two matched sticky ends together. 4. Without ligase, sticky-end interactions would be only transient hydrogen bonds. _Source: syllabus Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2 tools_
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