The role of DNA ligase in recombinant DNA technology is to

Question

Accepted Answer

B. {'text': 'Join the ends of two DNA fragments', 'label': 'B'}

1. Restriction enzymes cut DNA; DNA ligase joins DNA.
2. Ligase forms a phosphodiester bond between adjacent nucleotides on a cut DNA strand.
3. It seals the sugar-phosphate backbone of two matched sticky ends together.
4. Without ligase, sticky-end interactions would be only transient hydrogen bonds.
_Source: NCERT Class 12 Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2 tools_

Answer

{'text': 'Cut DNA at palindromic sites', 'label': 'A'}

Answer

{'text': 'Copy DNA into RNA', 'label': 'C'}

Answer

{'text': 'Digest single-stranded DNA', 'label': 'D'}