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After repeated PCR cycles, the gene of interest can be amplified to approximately:
A~100 copies
B~10⁶ copies
C~10²⁰ copies
D~10⁹ copies (1 billion) in ~30 cycles
Answer & Solution
Correct answer: D. ~10⁹ copies (1 billion) in ~30 cycles
Each cycle doubles the target → **2ⁿ copies after n cycles**. After ~30 cycles: ~2³⁰ ≈ **10⁹ (~1 billion) copies** — enough to clone, sequence, or use directly. NCERT explicitly states this.
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