Home › NEET UG › Biology › Biotechnology: Principles and Processes › After repeated PCR cycles, the gene of interest …
After repeated PCR cycles, the gene of interest can be amplified to approximately:
A~100 copies
B~10⁶ copies
C~10²⁰ copies
D~10⁹ copies (1 billion) in ~30 cycles
Answer & Solution
Correct answer: D. ~10⁹ copies (1 billion) in ~30 cycles
Each cycle doubles the target → **2ⁿ copies after n cycles**. After ~30 cycles: ~2³⁰ ≈ **10⁹ (~1 billion) copies** — enough to clone, sequence, or use directly. NCERT explicitly states this.
Related questions
Making multiple identical copies of a specific piece of DNA by linking it to an ori is terBiotechnology, per the European Federation of Biotechnology (EFB), is best defined asA "vector" in recombinant DNA technology serves toThe first restriction endonuclease that recognised a specific 6-base sequence and cut DNA Which of the following is NOT a step in genetically modifying an organism as per NCERT?A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting ofNucleases fall into two broad categories:The presence of an "origin of replication" is important because it