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CBSE Class 12 (Science) Biotechnology Applications — practice questions

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Restriction enzymes are best described as:In recombinant DNA technology, plasmids are commonly used as:The three sequential steps of one PCR cycle are:Taq DNA polymerase used in PCR is obtained from:In gel electrophoresis, DNA fragments move toward the:The standard PLANT cell transformation method uses:The first MAJOR recombinant DNA pharmaceutical, released commercially in 1982, was:Bt cotton is resistant to BOLLWORM pests because it carries a gene from:The power of any plant cell to grow into a whole plant is:Raising thousands of plants through tissue culture is called:Plants raised from one parent by tissue culture are called:Virus free plants can be raised from an infected plant using its:Plant cells stripped of their walls by digestion are called:Fusing protoplasts of two varieties gives a plant known as a:Rice enriched with vitamin A by genetic modification is:The Bt toxin used in pest resistant crops comes from a:Inside Bacillus thuringiensis the Bt toxin is stored as:The Bt protoxin becomes an active toxin because the insect gut is:Once activated the Bt toxin kills by acting on the insect:The genes cryIAc and cryIIAb protect cotton against:Tobacco roots are protected from a nematode by silencing its:Silencing a gene with a complementary double stranded RNA is:Human insulin is built from two polypeptide chains joined by:The extra stretch removed as pro-insulin matures is the:Human insulin was first produced by recombinant means in:Inserting a normal gene to correct an inherited defect is:The first clinical gene therapy treated a deficiency of:The ADA gene is carried into a patient's lymphocytes by a:Very low numbers of a pathogen can be detected early by:PCR is now used routinely to detect which virus?ELISA works on the principle of interaction between:In molecular diagnosis the labelled single strand used is called a:Most transgenic animals produced so far have been:Rosie, the first transgenic cow, gave milk enriched in human:Transgenic mice are being used to test the safety of the:Which body clears the safety of GM organisms in India?Using a country's bio-resources without authorisation is:How many documented varieties of Basmati are grown in India?Bt toxin is produced by a bacterium of which genus?Which of these is a Bt transgenic crop?Inside the bacterium, the Bt toxin exists as an:What converts the Bt protoxin into its active form?Most Bt toxins are specific to a particular:The Bt toxin is coded by a gene named:Which genes control the cotton bollworms?Which gene controls the corn borer?Silencing a specific mRNA using double-stranded RNA is called:Human insulin is made up of how many polypeptide chains?The two insulin chains are linked together by:In mammals, insulin is first synthesised as a:Which extra stretch is removed as insulin matures?Which company prepared the DNA sequences for human insulin?In which year was that done?The insulin chains were produced in plasmids of:The first clinical gene therapy was given in the year:The first gene therapy patient was a girl aged:Adenosine deaminase is crucial for the functioning of the:ELISA works on the principle of interaction between antigen and:What is the capacity of a single plant cell to regenerate a whole plant called?Plants grown from one parent by micro-propagation are genetically identical and called what?Which sugar serves as the carbon source in a tissue culture medium?Which pair of growth regulators is added to plant tissue culture medium?Which plant part is best for obtaining virus-free plants in culture?Any plant part removed and grown in a sterile test tube is called what?Plant cells with their walls digested away yield what structures?Golden rice is enriched with which vitamin?Pest-resistant GM crops mainly reduce reliance on which input?The Bt toxin gene is obtained from which bacterium?In what form does the Bt toxin exist inside the bacterium?What activates the Bt protoxin crystals inside an insect?Tobacco budworm and armyworm belong to which insect group?Which common insects are coleopterans, killed by some Bt proteins?Silencing of a specific mRNA by a complementary dsRNA is called what?In which group of organisms does RNAi act as cellular defence?The RNAi strategy protected the roots of which crop from a nematode?Which vector delivered nematode-specific genes into tobacco cells?About how many recombinant therapeutics are marketed in India?What links insulin chain A to chain B?Which stretch of pro-insulin is removed during maturation?Which company made human insulin chains in E. coli in 1983?How were separately made insulin chains A and B joined?In ADA gene therapy, which cells are cultured and given functional cDNA?Why do ADA patients need periodic infusion of engineered lymphocytes?A permanent cure for ADA deficiency needs the gene in which cells?In enzyme replacement therapy for ADA deficiency, what is injected?Which technique amplifies HIV nucleic acid for detection?A radioactive probe hybridised to cloned DNA is detected by what method?What fraction of existing transgenic animals are mice?Rosie's milk contained which human protein?A transgenic animal protein is used to treat which disease?Transgenic mice may replace which animals in testing polio vaccine safety?What is the advantage of toxicity testing in transgenic animals?What does GEAC stand for in India?Using bio-resources without authorisation or payment is called what?In 1997 an American company got a US patent on which Indian crop?About how many rice varieties are estimated to exist in India?