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AP Intermediate 2nd Year Biotechnology and its Applications — practice questions

Explore 11 free Biotechnology and its Applications questions in AP Intermediate 2nd Year Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and its Applications questions and exercises with answers

Showing questions 1–11 of 11.

The first restriction endonuclease that recognised a specific 6-base sequence and cut DNA wasRead the answer and worked solution →A "vector" in recombinant DNA technology serves toRead the answer and worked solution →Making multiple identical copies of a specific piece of DNA by linking it to an ori is termedRead the answer and worked solution →Bt toxin is produced by a bacterium of which genus?Read the answer and worked solution →Which of these is a Bt transgenic crop?Read the answer and worked solution →Inside the bacterium, the Bt toxin exists as an:Read the answer and worked solution →What converts the Bt protoxin into its active form?Read the answer and worked solution →Most Bt toxins are specific to a particular:Read the answer and worked solution →The Bt toxin is coded by a gene named:Read the answer and worked solution →Which genes control the cotton bollworms?Read the answer and worked solution →Which gene controls the corn borer?Read the answer and worked solution →

Worked practice questions

The first restriction endonuclease that recognised a specific 6-base sequence and cut DNA was

  1. EcoRI
  2. PstI
  3. BamHI
  4. Hind II
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Correct answer: D. Hind II

1. Your syllabus notes that two enzymes were isolated in 1963 in E. coli that restricted phage growth. 2. One added methyl groups; the other cut DNA (a "restriction endonuclease"). 3. The first restriction endonuclease with a sequence-specific cutting site was Hind II, characterised five years later. 4. Hind II recognises a specific sequence of six base pairs. _Source: syllabus Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2.1_

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A "vector" in recombinant DNA technology serves to

  1. Carry alien DNA into the host cell
  2. Add methyl groups to DNA
  3. Prevent replication of DNA
  4. Excise all restriction sites
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Correct answer: A. Carry alien DNA into the host cell

1. A vector in cloning transports an alien DNA fragment into a host organism. 2. syllabus draws an analogy with a mosquito acting as a biological vector. 3. Plasmids and bacteriophages are common cloning vectors. 4. A good vector has an ori, selectable marker and unique cloning sites for the insert. _Source: syllabus Biology, Ch 9 "Biotechnology: Principles and Processes", §9.1_

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Making multiple identical copies of a specific piece of DNA by linking it to an ori is termed

  1. Transcription
  2. Replication of the host genome
  3. Cloning
  4. Transformation
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Correct answer: C. Cloning

1. Cloning in the molecular-biology sense means producing many identical copies of a DNA template. 2. The alien DNA must be linked to an origin of replication or integrated into a chromosome. 3. Cohen and Boyer's work "cloned" an antibiotic-resistance gene in E. coli by 1972. 4. Transformation refers to entry of DNA into a host; transcription is DNA → RNA. _Source: syllabus Biology, Ch 9 "Biotechnology: Principles and Processes", §9.1_

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More Biology topics

Genetics and EvolutionReproduction in Organisms & PlantsBiotechnology and ApplicationsBiology in Human Welfare & HealthEcology and EnvironmentReproductive HealthOrganisms and PopulationsBiodiversity and Conservation