The first restriction endonuclease that recognised a specific 6-base s…

Question

The first restriction endonuclease that recognised a specific 6-base sequence and cut DNA was

Accepted Answer

D. {'text': 'Hind II', 'label': 'D'}

1. NCERT notes that two enzymes were isolated in 1963 in E. coli that restricted phage growth.
2. One added methyl groups; the other cut DNA (a "restriction endonuclease").
3. The first restriction endonuclease with a sequence-specific cutting site was Hind II, characterised five years later.
4. Hind II recognises a specific sequence of six base pairs.
_Source: NCERT Class 12 Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2.1_

Answer

{'text': 'EcoRI', 'label': 'A'}

Answer

{'text': 'PstI', 'label': 'B'}

Answer

{'text': 'BamHI', 'label': 'C'}