alleryWhich enzyme joins two cut DNA ends to make recombinant DNA?
ADNA ligase
BDNA primase
CDNA helicase
DRNA polymerase
Answer & Solution
Correct answer: A. DNA ligase
1. Restriction enzymes only cut; something must rejoin the pieces.
2. DNA ligase acts on cut DNA molecules and seals their ends.
3. So the vector and the foreign fragment are stitched by ligase.
_Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_
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