Apart from size, what distinguishes a vacuole from a vesicle?

1. Vesicles and vacuoles are both membrane-bound sacs that function in storage and transport. 2. Vacuoles are somewhat larger than vesicles, but size is only the coarse difference. 3. The subtle difference lies in what each membrane can do. 4. A vesicle membrane can fuse with the plasma membrane or with other membrane systems inside the cell. 5. The membrane of a vacuole does not fuse with the membranes of other cellular components. 6. Enzymes within plant vacuoles do break down macromolecules, but that is a plant feature rather than the membrane distinction asked for. 7. The distinguishing property is that a vesicle membrane can fuse with others. _Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 4 "Cell Structure", section 4.3 Eukaryotic Cells_