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Which of the following is NOT a step in classical DNA fingerprinting?
AIsolation of DNA, digestion with restriction endonucleases, electrophoresis
BHybridisation with labelled VNTR probe + autoradiography
CSouthern blotting onto nitrocellulose/nylon membrane
DSanger sequencing of every base pair
Answer & Solution
Correct answer: D. Sanger sequencing of every base pair
Classical Jeffreys DNA fingerprinting: isolate DNA → restriction digest → gel electrophoresis → **Southern blot** → hybridise with radiolabelled VNTR probe → **autoradiography**. Sanger sequencing is unrelated (used in HGP). Modern fingerprinting uses PCR with very small samples.
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