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In a transcription unit, the strand that has the polarity 3' to 5' and is used by RNA polymerase to direct RNA synthesis is called the:

ACoding strand
BTemplate strand
CSense strand
DAntisense promoter
Answer & Solution
Correct answer: B. Template strand
RNA polymerase synthesises 5' to 3', so the strand read must be 3' to 5'. This is the template (antisense) strand. The complementary 5' to 3' strand, identical to RNA except T for U, is the coding (sense) strand.
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