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In the Meselson-Stahl experiment, DNA extracted one generation after the shift from 15N to 14N medium showed which band pattern in CsCl gradient?
A{'text': 'A single heavy band', 'label': 'A'}
B{'text': 'A single intermediate band', 'label': 'B'}
C{'text': 'A single light band', 'label': 'C'}
D{'text': 'Equal heavy and light bands', 'label': 'D'}
Answer & Solution
Correct answer: B. {'text': 'A single intermediate band', 'label': 'B'}
After one generation in 14N, every duplex was a 15N/14N hybrid, banding at intermediate density. The result rules out conservative replication (which would give heavy + light bands at gen-1) and confirms semi-conservative replication.
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