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CUET UG Biotechnology and Applications — practice questions

Explore 112 free Biotechnology and Applications questions in CUET UG Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 1–30 of 112.

Which enzyme joins two cut DNA ends to make recombinant DNA?Read the answer and worked solution →The first restriction endonuclease to be isolated was:Read the answer and worked solution →Hind II cuts DNA on recognising a specific sequence of:Read the answer and worked solution →Restriction enzymes are popularly called:Read the answer and worked solution →Nucleases that remove nucleotides from DNA ends are:Read the answer and worked solution →In gel electrophoresis DNA fragments move towards the:Read the answer and worked solution →Which matrix is most commonly used to separate DNA fragments?Read the answer and worked solution →In an agarose gel the fragment that travels farthest is the:Read the answer and worked solution →Separated DNA bands become visible after staining with:Read the answer and worked solution →Cutting a stained DNA band out of the gel and recovering it is:Read the answer and worked solution →The vector sequence from which replication begins is the:Read the answer and worked solution →An antibiotic resistance gene on a vector serves mainly as a:Read the answer and worked solution →A foreign gene ligated at the BamH I site of pBR322 destroys:Read the answer and worked solution →Colonies whose beta galactosidase gene carries an insert appear:Read the answer and worked solution →Loss of gene function caused by a DNA insert inside it is:Read the answer and worked solution →Which plasmid was reworked into a cloning vector for plants?Read the answer and worked solution →Bacterial cells are made competent by treating them with a divalent:Read the answer and worked solution →Recombinant DNA uptake is helped by a brief heat shock at:Read the answer and worked solution →Bombarding plant cells with DNA coated gold particles is called:Read the answer and worked solution →Micro-injection puts recombinant DNA directly into the:Read the answer and worked solution →PCR amplifies a chosen segment using two sets of:Read the answer and worked solution →The thermostable polymerase used in PCR is taken from:Read the answer and worked solution →Repeated cycles of PCR can amplify a segment about:Read the answer and worked solution →Which enzyme is used to open plant cells before DNA isolation?Read the answer and worked solution →Purified DNA finally precipitates out on adding chilled:Read the answer and worked solution →Separation and purification of the final product is called:Read the answer and worked solution →The power of any plant cell to grow into a whole plant is:Read the answer and worked solution →Raising thousands of plants through tissue culture is called:Read the answer and worked solution →Plants raised from one parent by tissue culture are called:Read the answer and worked solution →Virus free plants can be raised from an infected plant using its:Read the answer and worked solution →

Worked practice questions

Which enzyme joins two cut DNA ends to make recombinant DNA?

  1. DNA ligase
  2. DNA primase
  3. DNA helicase
  4. RNA polymerase
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Correct answer: A. DNA ligase

1. Restriction enzymes only cut; something must rejoin the pieces. 2. DNA ligase acts on cut DNA molecules and seals their ends. 3. So the vector and the foreign fragment are stitched by ligase. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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The first restriction endonuclease to be isolated was:

  1. Pst I
  2. Hind II
  3. EcoR I
  4. BamH I
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Correct answer: B. Hind II

1. Two enzymes restricting phage growth were found in 1963. 2. The one that cut DNA was named restriction endonuclease. 3. Hind II was the first such enzyme characterised. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Hind II cuts DNA on recognising a specific sequence of:

  1. Six base pairs
  2. Two base pairs
  3. Ten base pairs
  4. One base pair
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Correct answer: A. Six base pairs

1. Hind II always cuts at a particular point. 2. That point is fixed by a recognition sequence. 3. The recognition sequence is six base pairs long. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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