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AP Intermediate 2nd Year Biotechnology and Applications — practice questions — page 5

Explore 167 free Biotechnology and Applications questions in AP Intermediate 2nd Year Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 121–150 of 167.

The Bt protoxin becomes an active toxin because the insect gut is:Read the answer and worked solution →Once activated the Bt toxin kills by acting on the insect:Read the answer and worked solution →Tobacco roots are protected from a nematode by silencing its:Read the answer and worked solution →ELISA works on the principle of interaction between:Read the answer and worked solution →In molecular diagnosis the labelled single strand used is called a:Read the answer and worked solution →Using a country's bio-resources without authorisation is:Read the answer and worked solution →How many documented varieties of Basmati are grown in India?Read the answer and worked solution →Order the steps of Bt toxin actionRead the answer and worked solution →The ADA gene is carried into a patient's lymphocytes by a:Read the answer and worked solution →Very low numbers of a pathogen can be detected early by:Read the answer and worked solution →True or false about restriction enzymesRead the answer and worked solution →Which engineering does each job belong to?Read the answer and worked solution →Recombinant DNA uptake is helped by a brief heat shock at:Read the answer and worked solution →The first restriction endonuclease to be isolated was:Read the answer and worked solution →Restriction enzymes are best described as:Read the answer and worked solution →Cutting a stained DNA band out of the gel and recovering it is:Read the answer and worked solution →Human insulin is built from two polypeptide chains joined by:Read the answer and worked solution →The extra stretch removed as pro-insulin matures is the:Read the answer and worked solution →Human insulin was first produced by recombinant means in:Read the answer and worked solution →Inserting a normal gene to correct an inherited defect is:Read the answer and worked solution →The first clinical gene therapy treated a deficiency of:Read the answer and worked solution →Nucleases that remove nucleotides from DNA ends are:Read the answer and worked solution →Spot the one that does not fitRead the answer and worked solution →In gel electrophoresis, DNA fragments move toward the:Read the answer and worked solution →Separated DNA bands become visible after staining with:Read the answer and worked solution →Loss of gene function caused by a DNA insert inside it is:Read the answer and worked solution →Which plasmid was reworked into a cloning vector for plants?Read the answer and worked solution →Match each biotechnology term to its meaning.Read the answer and worked solution →True or false about transgenic workRead the answer and worked solution →Fill the gaps in the cloning storyRead the answer and worked solution →

Worked practice questions

The Bt protoxin becomes an active toxin because the insect gut is:

  1. Acidic
  2. Neutral
  3. Anoxic
  4. Alkaline
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Correct answer: D. Alkaline

1. The crystal has to dissolve before it can act. 2. The gut of the insect is alkaline. 3. That alkaline pH solubilises the crystals. _Source: syllabus Biology, Ch 10 'Biotechnology and its Applications'_

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Once activated the Bt toxin kills by acting on the insect:

  1. Midgut cells
  2. Wing muscles
  3. Nerve cords
  4. Egg cases
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Correct answer: A. Midgut cells

1. The active toxin binds the midgut epithelial surface. 2. It creates pores in those cells. 3. The cells swell and burst and the insect dies. _Source: syllabus Biology, Ch 10 'Biotechnology and its Applications'_

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Tobacco roots are protected from a nematode by silencing its:

  1. Transfer RNA
  2. Chromosomal DNA
  3. Messenger RNA
  4. Ribosomal RNA
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Correct answer: C. Messenger RNA

1. The host is made to produce sense and anti-sense RNA. 2. These pair into a double stranded RNA molecule. 3. That double strand silences the nematode messenger RNA. _Source: syllabus Biology, Ch 10 'Biotechnology and its Applications'_

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