alleryStart learning →The widely used E. coli cloning vector discussed for selection is which?
- pBR322
- pUC1
- M13mp
- lambda
Show answer and solution
Correct answer: A. pBR322
pBR322 has ampR and tetR genes and several unique restriction sites for cloning.
AP EAMCET Botany Unit 12: Biotechnology — practice questions — page 2
Explore 80 free Botany Unit 12: Biotechnology questions in AP EAMCET Biology. Open each question or exercise to read its content, reference answers and available explanation.
Practise Botany Unit 12: Biotechnology with Quill →Botany Unit 12: Biotechnology questions and exercises with answers
Showing questions 31–60 of 80.
The widely used E. coli cloning vector discussed for selection is which?Read the answer and worked solution →A foreign DNA inserted at the BamH I site of pBR322 inactivates which gene?Read the answer and worked solution →A colony that grows on ampicillin but not on tetracycline is what?Read the answer and worked solution →Non-recombinant colonies on a chromogenic plate appear what colour?Read the answer and worked solution →Which bacterium's Ti plasmid is modified as a plant cloning vector?Read the answer and worked solution →Which vectors, once disarmed, deliver genes into animal cells?Read the answer and worked solution →Bacterial cells are made competent for DNA uptake by treating with which ion?Read the answer and worked solution →The heat shock given to competent bacteria is at what temperature?Read the answer and worked solution →Direct injection of recombinant DNA into an animal cell nucleus is called what?Read the answer and worked solution →A gene gun for plant cells uses particles of which metals?Read the answer and worked solution →Which enzyme is used to break open bacterial cells for DNA isolation?Read the answer and worked solution →Plant cell walls are broken down for DNA isolation using which enzyme?Read the answer and worked solution →Which enzyme removes contaminating RNA during DNA purification?Read the answer and worked solution →Which reagent precipitates purified DNA as fine threads?Read the answer and worked solution →PCR uses how many sets of primers to amplify a gene?Read the answer and worked solution →Which is the correct order of steps in each PCR cycle?Read the answer and worked solution →The thermostable DNA polymerase used in PCR comes from which bacterium?Read the answer and worked solution →Repeated PCR can amplify a DNA segment to about how many copies?Read the answer and worked solution →Why is a thermostable polymerase needed in PCR?Read the answer and worked solution →A cell growing in continuous culture is kept in which phase?Read the answer and worked solution →A protein from a gene expressed in a heterologous host is called what?Read the answer and worked solution →The most commonly used bioreactors are of which type?Read the answer and worked solution →Separation and purification of a product together form what stage?Read the answer and worked solution →Totipotency is the capacity of a plant cell or explant to do what?Read the answer and worked solution →Genetically identical plants produced by micropropagation are called what?Read the answer and worked solution →Which plant part is free of virus even in an infected plant?Read the answer and worked solution →Fusion of protoplasts from two plant varieties gives what?Read the answer and worked solution →The tomato and potato protoplast fusion product is known as what?Read the answer and worked solution →Golden rice is enriched with which vitamin?Read the answer and worked solution →Which bacterium produces the insecticidal Bt toxin?Read the answer and worked solution →
Worked practice questions
A foreign DNA inserted at the BamH I site of pBR322 inactivates which gene?
- Ampicillin resistance
- Replication gene rop
- Kanamycin resistance
- Tetracycline resistance
Show answer and solution
Correct answer: D. Tetracycline resistance
The BamH I site lies in the tetracycline resistance gene, so insertion makes recombinants lose tetracycline resistance.
A colony that grows on ampicillin but not on tetracycline is what?
- Recombinant
- Non-transformant
- Non-recombinant
- Wild-type host
Show answer and solution
Correct answer: A. Recombinant
Insertion into the tetracycline gene destroys tetracycline resistance while ampicillin resistance stays, marking a recombinant.
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