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AP EAMCET Botany Unit 12: Biotechnology — practice questions

Explore 80 free Botany Unit 12: Biotechnology questions in AP EAMCET Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Botany Unit 12: Biotechnology questions and exercises with answers

Showing questions 1–30 of 80.

Which scientists made the first recombinant DNA molecule in 1972?Read the answer and worked solution →The first recombinant DNA used a plasmid of which bacterium?Read the answer and worked solution →Restriction enzymes are often called what in genetic engineering?Read the answer and worked solution →Which enzyme joins the cut ends of DNA fragments?Read the answer and worked solution →A cut-and-joined DNA made in vitro from different sources is known as what?Read the answer and worked solution →Which sequence in a chromosome starts DNA replication?Read the answer and worked solution →Which pair of core techniques gave birth to modern biotechnology?Read the answer and worked solution →Bioprocess engineering mainly ensures what?Read the answer and worked solution →Which enzyme class cuts DNA at specific positions within the molecule?Read the answer and worked solution →Exonucleases differ from endonucleases because they do what?Read the answer and worked solution →The first restriction endonuclease isolated and characterised was which one?Read the answer and worked solution →Hind II always cuts DNA by recognising a sequence of how many base pairs?Read the answer and worked solution →In the name EcoRI, the capital letter E is taken from what?Read the answer and worked solution →In the name EcoRI, the Roman numeral I indicates what?Read the answer and worked solution →EcoRI is isolated from which organism?Read the answer and worked solution →Restriction enzymes recognise which type of nucleotide sequence?Read the answer and worked solution →Which sequence is the recognition site of EcoRI?Read the answer and worked solution →The overhanging single-stranded ends left after restriction cuts are called what?Read the answer and worked solution →Why are sticky ends useful for ligase action?Read the answer and worked solution →Vector and source DNA must be cut with what?Read the answer and worked solution →In gel electrophoresis, DNA fragments move toward which electrode?Read the answer and worked solution →Which natural polymer from seaweeds is the usual matrix for DNA electrophoresis?Read the answer and worked solution →In an agarose gel, smaller DNA fragments move how?Read the answer and worked solution →DNA bands in a gel are stained with which compound?Read the answer and worked solution →Ethidium bromide stained DNA bands are seen under which light?Read the answer and worked solution →Extracting separated DNA bands from the agarose gel is called what?Read the answer and worked solution →Which feature of a vector controls the copy number of linked DNA?Read the answer and worked solution →What is the role of a selectable marker in a vector?Read the answer and worked solution →Which gene is a useful selectable marker for E. coli?Read the answer and worked solution →Why must a vector have very few recognition sites?Read the answer and worked solution →

Worked practice questions

Which scientists made the first recombinant DNA molecule in 1972?

  1. Jacob and Monod
  2. Watson and Crick
  3. Meselson and Stahl
  4. Cohen and Boyer
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Correct answer: D. Cohen and Boyer

Stanley Cohen and Herbert Boyer joined an antibiotic resistance gene to a plasmid in 1972, creating the first recombinant DNA.

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The first recombinant DNA used a plasmid of which bacterium?

  1. Bacillus thuringiensis
  2. Agrobacterium tumifaciens
  3. Thermus aquaticus
  4. Salmonella typhimurium
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Correct answer: D. Salmonella typhimurium

The antibiotic resistance gene was linked to a plasmid taken from Salmonella typhimurium, and later cloned in the related E. coli.

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Restriction enzymes are often called what in genetic engineering?

  1. Molecular glue
  2. Molecular carriers
  3. Molecular scissors
  4. Molecular clocks
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Correct answer: C. Molecular scissors

They cut DNA at specific recognition sequences, so they act like scissors for DNA.

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