Why must the DNA polymerase in PCR be thermostable?
AIt must survive the denaturation heat
BIt must cut DNA at high temperature
CIt must join primers to the vector
DIt must break open the cell wall
Answer & Solution
Correct answer: A. It must survive the denaturation heat
Each cycle heats DNA to separate the strands, and only a heat-stable polymerase stays active across cycles.
Keep practising Biotechnology: Principles and Processes
Browse all Biotechnology: Principles and Processes questions for NEET UG, or explore more Biology topics.
Related questions
Why is a buffer used during gel electrophoresis?Proteins in a gel are commonly stained with which dye?Unlike vertical polyacrylamide gels, how are agarose gels typically run?Which gel has very high resolving power for small DNA fragments of 5 to 500 bp?Variation among individuals in restriction fragment lengths of genomic DNA is called what?A short plasmid stretch rich in restriction recognition sites is called what?Which type of restriction enzyme cuts DNA at sites remote from its recognition site?Typical type II restriction enzymes usually need which cofactor alone?