In a standard IVF protocol, the ideal ratio of sperm to egg mixed together in the petri dish is approximately:
A1 sperm to one egg
B1,000 sperm to one egg
C75,000 sperm to one egg
D1 million sperm to one egg
Answer & Solution
Correct answer: C. 75,000 sperm to one egg
1. During standard IVF, eggs and prepared sperm are combined together in a petri dish.
2. Because natural fertilization already requires many sperm to break down the oocyte's protective layers, a large excess is still used in vitro.
3. Seminal fluid is washed away before mixing because it contains FPP, a peptide that can block sperm capacitation at high concentration.
4. The eggs and prepared sperm are then combined at a specific, deliberately high ratio.
5. 1 sperm to one egg and 1,000 sperm to one egg are both different figures a reader might guess from memory, but neither matches the specific figure given here.
6. That ratio is 75,000 sperm to one egg; if sperm quality is very poor, intracytoplasmic sperm injection (ICSI) is used instead.
_Source: OpenStax Anatomy and Physiology (CC BY 4.0), Ch 28 "Development and Inheritance", p.1322-1323 §In Vitro Fertilization_
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