The enzyme phospholipase C cleaves PIP2 into two separate second messengers. What happens to each of these two products?

1. Phospholipase C cleaves PIP2 to form diacylglycerol (DAG) and inositol triphosphate (IP3), both of which serve as second messengers. 2. DAG remains in the plasma membrane and activates protein kinase C, or PKC. 3. PKC then phosphorylates serine and threonine residues in its target proteins. 4. IP3, in contrast, diffuses into the cytoplasm and binds to ligand-gated calcium channels in the endoplasmic reticulum. 5. This IP3 binding releases calcium ions that continue the signal cascade. 6. The option that swaps the roles of DAG and IP3 reverses which product stays in the membrane and which one diffuses into the cytoplasm. 7. Neither product diffuses into the nucleus to bind DNA directly, so that option misdescribes their site of action. 8. Neither product forms a new ion channel; IP3 instead opens an existing ligand-gated calcium channel on the endoplasmic reticulum. _Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 9 "Cell Communication", section Second Messengers_