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In which compartment does the citric acid cycle take place?

AIn the matrix of mitochondria
BIn the intermembrane space
CIn the outer mitochondrial membrane
DIn the cytoplasm outside
Answer & Solution
Correct answer: A. In the matrix of mitochondria
1. Glycolysis runs in the cytoplasm, but the next stages move inside the mitochondrion. 2. The conversion of pyruvate to acetyl coenzyme A takes place in the matrix of mitochondria. 3. The citric acid cycle takes place in the same compartment, the matrix. 4. The intermembrane space is where protons collect later, so it hosts no cycle enzymes. _Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 7 "Cellular Respiration", section 7.3 Oxidation of Pyruvate and the Citric Acid Cycle_
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