What stalls the polymerase during rho-independent termination?
AA protein that tracks along the new mRNA
BA run of G nucleotides on the template
CA hairpin formed within the new mRNA
DA ribosome translating the same mRNA
Answer & Solution
Correct answer: C. A hairpin formed within the new mRNA
1. Rho-independent termination is controlled by sequences in the DNA template rather than by a protein.
2. The transcript folds back on itself and its complementary nucleotides pair, forming a stable hairpin.
3. That hairpin stalls the polymerase, and the weak pairing that follows lets the core enzyme break away with the new transcript.
4. A protein tracking along the mRNA is rho itself, which defines the other kind of termination.
_Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 15 "Genes and Proteins", section 15.2 Prokaryotic Transcription_
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