alleryStart learning →In rho-dependent termination, what makes the polymerase stall so that rho can catch up with it?
AA hairpin that forms within the new mRNA
BA run of G nucleotides on the DNA template
CA region rich in A and T on the template
DA release factor entering the polymerase
Answer & Solution
Correct answer: B. A run of G nucleotides on the DNA template
1. The rho protein tracks along behind the polymerase on the growing mRNA chain.
2. Near the end of the gene the polymerase meets a run of G nucleotides on the DNA template and stalls.
3. Because the polymerase has stopped, rho collides with it.
4. That interaction releases the mRNA from the transcription bubble.
5. A hairpin in the transcript stalls the polymerase in the rho-independent route, so it belongs to the other mechanism.
6. Release factors act in translation termination, not transcription termination.
_Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 15 "Genes and Proteins", section 15.2 Prokaryotic Transcription_
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