NEET UG Biotechnology Applications — practice questions
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Practice NEET UG Biotechnology Applications in the app →The three basic steps in genetically modifying an organism are:Restriction endonucleases are nucleases that:In the name 'EcoRI', the components stand for:Restriction enzymes cut DNA in such a way that they produce 'sticky ends'. This is useful because:
The cloning vector pBR3In biolistics (gene gun) used for plant transformation:To isolate DNA from bacterial cells, the cell wall is digested by:To remove contaminating RNA and protein during DNA isolation, the following enzymes are used respectively:
The three steps that makAfter repeated PCR cycles, the gene of interest can be amplified to approximately:Some uses of PCR amplification include:Downstream processing in industrial biotechnology refers to:Recombinant DNA can be inserted into a host cell where its protein product is produced. Such a protein expressIn a palindromic DNA sequence:Which of the following is the *correct* sequence of steps in basic recombinant DNA technology?Restriction enzymes are best described as:In recombinant DNA technology, plasmids are commonly used as:The three sequential steps of one PCR cycle are:Taq DNA polymerase used in PCR is obtained from:In gel electrophoresis, DNA fragments move toward the:The standard PLANT cell transformation method uses:The first MAJOR recombinant DNA pharmaceutical, released commercially in 1982, was:Bt cotton is resistant to BOLLWORM pests because it carries a gene from:In India, the regulatory body for releasing genetically modified organisms (GMOs) is:The two core techniques that enabled modern biotechnology as per NCERT Ch 9 areThe first construction of a recombinant DNA molecule (1972) was achieved byThe naming convention of the restriction enzyme EcoRI comes fromA recognition sequence acted on by a restriction endonuclease is characteristicallyThe "sticky ends" produced by a restriction endonuclease areThe role of DNA ligase in recombinant DNA technology is toA plasmid used as a vector in recombinant DNA work isThe presence of an "origin of replication" is important because itNucleases fall into two broad categories:A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting ofWhich of the following is NOT a step in genetically modifying an organism as per NCERT?The first restriction endonuclease that recognised a specific 6-base sequence and cut DNA wasA "vector" in recombinant DNA technology serves toBiotechnology, per the European Federation of Biotechnology (EFB), is best defined asMaking multiple identical copies of a specific piece of DNA by linking it to an ori is termedThe power of any plant cell to grow into a whole plant is:Raising thousands of plants through tissue culture is called:Plants raised from one parent by tissue culture are called:Virus free plants can be raised from an infected plant using its:Plant cells stripped of their walls by digestion are called:Fusing protoplasts of two varieties gives a plant known as a:Rice enriched with vitamin A by genetic modification is:The Bt toxin used in pest resistant crops comes from a:Inside Bacillus thuringiensis the Bt toxin is stored as:The Bt protoxin becomes an active toxin because the insect gut is:Once activated the Bt toxin kills by acting on the insect:The genes cryIAc and cryIIAb protect cotton against:Tobacco roots are protected from a nematode by silencing its:Silencing a gene with a complementary double stranded RNA is:Human insulin is built from two polypeptide chains joined by:The extra stretch removed as pro-insulin matures is the:Human insulin was first produced by recombinant means in:Inserting a normal gene to correct an inherited defect is:The first clinical gene therapy treated a deficiency of:The ADA gene is carried into a patient's lymphocytes by a:Very low numbers of a pathogen can be detected early by:PCR is now used routinely to detect which virus?ELISA works on the principle of interaction between:In molecular diagnosis the labelled single strand used is called a:Most transgenic animals produced so far have been:Rosie, the first transgenic cow, gave milk enriched in human:Transgenic mice are being used to test the safety of the:Which body clears the safety of GM organisms in India?Using a country's bio-resources without authorisation is:How many documented varieties of Basmati are grown in India?Bt toxin is produced by a bacterium of which genus?The full name of that bacterium is Bacillus:Which of these is a Bt transgenic crop?Inside the bacterium, the Bt toxin exists as an:What converts the Bt protoxin into its active form?Most Bt toxins are specific to a particular:The Bt toxin is coded by a gene named:Which genes control the cotton bollworms?Which gene controls the corn borer?Silencing a specific mRNA using double-stranded RNA is called:Human insulin is made up of how many polypeptide chains?The two insulin chains are linked together by:In mammals, insulin is first synthesised as a:Which extra stretch is removed as insulin matures?Which company prepared the DNA sequences for human insulin?In which year was that done?The insulin chains were produced in plasmids of:The first clinical gene therapy was given in the year:The first gene therapy patient was a girl aged:That girl suffered a deficiency of:Adenosine deaminase is crucial for the functioning of the:ELISA works on the principle of interaction between antigen and: