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A recognition sequence acted on by a restriction endonuclease is characteristically
A{'text': 'A single-stranded RNA motif', 'label': 'A'}
B{'text': 'A random sequence of any length', 'label': 'B'}
C{'text': 'A protein-coding gene only', 'label': 'C'}
D{'text': 'A palindromic double-stranded DNA sequence', 'label': 'D'}
Answer & Solution
Correct answer: D. {'text': 'A palindromic double-stranded DNA sequence', 'label': 'D'}
1. Restriction endonucleases recognise a specific double-stranded DNA sequence.
2. That sequence is palindromic: reads the same 5' → 3' on both strands.
3. Example: 5'-GAATTC-3' / 3'-CTTAAG-5' recognised by EcoRI.
4. The enzyme cuts a little away from the palindrome centre, leaving sticky ends.
_Source: NCERT Class 12 Biology, Ch 9 "Biotechnology: Principles and Processes", §9.2.1_
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