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Which of the following is the *correct* sequence of steps in basic recombinant DNA technology?
AInsertion → Cutting → Isolation → Selection → Expression
BExpression → Cutting → Isolation → Selection
CIsolation of DNA → Cutting with restriction enzyme → Insertion into vector → Transformation into host → Selection of recombinants → Expression and downstream processing
DRandom order — no fixed sequence
Answer & Solution
Correct answer: C. Isolation of DNA → Cutting with restriction enzyme → Insertion into vector → Transformation into host → Selection of recombinants → Expression and downstream processing
Standard rDNA workflow: **(1) Isolate DNA** → **(2) Cut with restriction enzyme** → **(3) Ligate insert into vector** → **(4) Transform host (competent E. coli)** → **(5) Select transformants (selectable marker / blue-white)** → **(6) Culture, induce expression, downstream processing**.
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