Home › NEET UG › Biology › Biotechnology: Principles and Processes › Which of the following is the *correct* sequence…
Which of the following is the *correct* sequence of steps in basic recombinant DNA technology?
AInsertion → Cutting → Isolation → Selection → Expression
BExpression → Cutting → Isolation → Selection
CIsolation of DNA → Cutting with restriction enzyme → Insertion into vector → Transformation into host → Selection of recombinants → Expression and downstream processing
DRandom order — no fixed sequence
Answer & Solution
Correct answer: C. Isolation of DNA → Cutting with restriction enzyme → Insertion into vector → Transformation into host → Selection of recombinants → Expression and downstream processing
Standard rDNA workflow: **(1) Isolate DNA** → **(2) Cut with restriction enzyme** → **(3) Ligate insert into vector** → **(4) Transform host (competent E. coli)** → **(5) Select transformants (selectable marker / blue-white)** → **(6) Culture, induce expression, downstream processing**.
Related questions
Making multiple identical copies of a specific piece of DNA by linking it to an ori is terBiotechnology, per the European Federation of Biotechnology (EFB), is best defined asA "vector" in recombinant DNA technology serves toThe first restriction endonuclease that recognised a specific 6-base sequence and cut DNA Which of the following is NOT a step in genetically modifying an organism as per NCERT?A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting ofNucleases fall into two broad categories:The presence of an "origin of replication" is important because it