Home › NEET UG › Biology › Biotechnology: Principles and Processes ›  The three steps that make up a single cycle of PCR are:
AInitiation, elongation, termination
BDenaturation, primer annealing, extension (primer elongation by DNA polymerase)
CAnnealing, extension, ligation
DTranscription, translation, replication
Answer & Solution
Correct answer: B. Denaturation, primer annealing, extension (primer elongation by DNA polymerase)
One PCR cycle = **(i) Denaturation** (~94°C, dsDNA → ssDNA), **(ii) Primer annealing** (~55°C, primers bind complementary template), **(iii) Extension** (~72°C, **Taq polymerase** elongates from primers). Repeated 25–35 times → exponential amplification (~1 billion copies).
Related questions
Making multiple identical copies of a specific piece of DNA by linking it to an ori is terBiotechnology, per the European Federation of Biotechnology (EFB), is best defined asA "vector" in recombinant DNA technology serves toThe first restriction endonuclease that recognised a specific 6-base sequence and cut DNA Which of the following is NOT a step in genetically modifying an organism as per NCERT?A restriction enzyme cutting 5'-G|AATTC-3' leaves overhangs consisting ofNucleases fall into two broad categories:The presence of an "origin of replication" is important because it