Home › NEET UG › Biology › Biotechnology Applications ›  The three steps that make up a single cycle of PCR are:
AInitiation, elongation, termination
BDenaturation, primer annealing, extension (primer elongation by DNA polymerase)
CAnnealing, extension, ligation
DTranscription, translation, replication
Answer & Solution
Correct answer: B. Denaturation, primer annealing, extension (primer elongation by DNA polymerase)
One PCR cycle = **(i) Denaturation** (~94°C, dsDNA → ssDNA), **(ii) Primer annealing** (~55°C, primers bind complementary template), **(iii) Extension** (~72°C, **Taq polymerase** elongates from primers). Repeated 25–35 times → exponential amplification (~1 billion copies).
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