Home › NEET UG › Biology › Biotechnology Applications ›  The cloning vector pBR322 contains all of the following features EXCEPT:
AAmpicillin resistance (amp^R) and tetracycline resistance (tet^R) genes as selectable markers
BThe lacZ gene for blue-white screening
CMultiple recognition sites for restriction enzymes (HindIII, EcoRI, BamHI, SalI, PvuII, PstI, ClaI)
DOrigin of replication (ori)
Answer & Solution
Correct answer: B. The lacZ gene for blue-white screening
**pBR322** has **ori, amp^R, tet^R, and single restriction sites** for many enzymes — but does **not** carry **lacZ** (that's in the **pUC** family used for **blue-white screening**). In pBR322, recombinants are selected by **insertional inactivation of tet^R** (still ampicillin-resistant but no longer tetracycline-resistant).
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