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Restriction enzymes cut DNA in such a way that they produce 'sticky ends'. This is useful because:
ASticky ends prevent any further enzymatic reaction
BThe fragments cannot rejoin and are easily lost
CThe complementary single-stranded overhangs base-pair (H-bond) with similarly cut fragments, facilitating ligation by DNA ligase
DThey allow exonuclease digestion
Answer & Solution
Correct answer: C. The complementary single-stranded overhangs base-pair (H-bond) with similarly cut fragments, facilitating ligation by DNA ligase
Sticky ends form **H-bonded base pairs** with complementary overhangs from any DNA cut by the same enzyme — so vector + insert from same enzyme can be **joined by DNA ligase** to create recombinant DNA. The cut sites must match.
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