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Restriction endonucleases are nucleases that:
ACut DNA at specific recognition sequences within the molecule (palindromic, typically 4–8 bp)
BLigate two DNA fragments
CRemove nucleotides from the ends of DNA (= exonucleases)
DSynthesise new DNA strands
Answer & Solution
Correct answer: A. Cut DNA at specific recognition sequences within the molecule (palindromic, typically 4–8 bp)
**Restriction endonucleases** make cuts at specific **palindromic recognition sequences** within DNA. **Exonucleases** chew from the ends. Hundreds (>900) are known from >230 bacterial strains. Bacteria use them to defend against invading phage DNA.
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