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Which step is NOT part of the classical DNA-fingerprinting workflow as described in NCERT?
A{'text': 'Restriction digestion of isolated DNA', 'label': 'A'}
B{'text': 'Reverse transcription of fragments to cDNA', 'label': 'B'}
C{'text': 'Electrophoretic separation of fragments', 'label': 'C'}
D{'text': 'Southern blot with VNTR probe', 'label': 'D'}
Answer & Solution
Correct answer: B. {'text': 'Reverse transcription of fragments to cDNA', 'label': 'B'}
The classical Jeffreys method runs DNA isolation, restriction digestion, gel electrophoresis, Southern blotting to nylon/nitrocellulose, hybridisation with radiolabelled VNTR probe and autoradiography. Reverse transcription is not part of the workflow.
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