alleryWhich step is NOT part of the classical DNA-fingerprinting workflow as described in your syllabus?
ARestriction digestion of isolated DNA
BReverse transcription of fragments to cDNA
CElectrophoretic separation of fragments
DSouthern blot with VNTR probe
Answer & Solution
Correct answer: B. Reverse transcription of fragments to cDNA
The classical Jeffreys method runs DNA isolation, restriction digestion, gel electrophoresis, Southern blotting to nylon/nitrocellulose, hybridisation with radiolabelled VNTR probe and autoradiography.
Reverse transcription is not part of the workflow.
Keep practising Unit 6A: DNA and RNA Structure & Replication
Browse all Unit 6A: DNA and RNA Structure & Replication questions for AP Biology, or explore more Biology topics.
Related questions
In eukaryotes, primary mRNA transcript becomes mature mRNA afterThe 'one gene - one polypeptide' concept was proposed byIn prokaryotes, the small ribosomal subunit recognises the mRNA initiation site via theIn humans, the percentage of A in a DNA sample is 30 %. By Chargaff's rule, the percentageTranscription differs from replication in that itThe genetic code is described as degenerate becauseOkazaki fragments are formed on theDuring DNA replication, the enzyme that unwinds the double helix is