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In the structural gene of a transcription unit, the **template strand** has polarity:
A5'→3'
B3'→5'
CEither, randomly
DBoth 5' and 3' simultaneously
Answer & Solution
Correct answer: B. 3'→5'
The template (antisense) strand runs 3'→5' (so that RNA polymerase, which synthesises mRNA 5'→3', can read it). The other DNA strand (coding/sense strand, 5'→3') has the same sequence as the mRNA (T replaced by U).
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