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The semiconservative nature of DNA replication was experimentally proved by:

AWatson and Crick
BMeselson and Stahl (1958)
CHershey and Chase
DAvery, MacLeod and McCarty
Answer & Solution
Correct answer: B. Meselson and Stahl (1958)
Meselson and Stahl (1958) cultured *E. coli* on ¹⁵N medium, transferred to ¹⁴N, and used density-gradient centrifugation in CsCl. After one generation, a single intermediate-density band appeared (one heavy, one light strand) — confirming semiconservative replication.
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