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During transcription, the strand of DNA actually read by RNA polymerase is
Athe coding strand (5′→3′)
Bneither — RNA polymerase reads its own RNA
Cthe template strand (3′→5′)
Dboth strands simultaneously
Answer & Solution
Correct answer: C. the template strand (3′→5′)
RNA polymerase reads the template strand in the 3′→5′ direction and builds the new RNA in the 5′→3′ direction. The other strand (with the same sequence as the RNA, except T not U) is called the coding strand by convention.
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