TS EAMCET Biotechnology: Principles and Processes — practice questions
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Practice TS EAMCET Biotechnology: Principles and Processes in the app →To isolate DNA from bacterial cells, the cell wall is digested by:After repeated PCR cycles, the gene of interest can be amplified to approximately:In which year did Cohen and Boyer construct the first recombinant DNA?Which enzymes act as molecular scissors that cut DNA at specific sites?Which enzyme joins cut DNA fragments end to end to make recombinant DNA?What do Roman numerals in a restriction enzyme name show?How do exonucleases differ from endonucleases?Which was the first restriction endonuclease whose action depended on a specific sequence?Restriction endonucleases usually recognise what kind of DNA sequence?What is a palindrome in DNA?What are the overhanging single-stranded ends left by restriction enzymes called?Why are sticky ends useful for recombinant DNA?Which technique separates DNA fragments cut by restriction enzymes?During gel electrophoresis, DNA fragments move towards which electrode?The agarose used for electrophoresis gel is extracted from what?In an agarose gel, which DNA fragments travel the farthest?Which compound stains DNA bands so they glow orange under UV light?What is elution in the preparation of DNA fragments?What is the function of the origin of replication (ori) in a vector?What is the role of a selectable marker in a cloning vector?Which antibiotic resistance gene is a useful selectable marker for E. coli vectors?What is transformation in genetic engineering?Why should a vector have only one site for an enzyme?A pBR322 clone with an insert at the BamH I site will grow on which medium?On a chromogenic plate, blue colonies show that the plasmid has what?Which plant pathogen is used to deliver T-DNA into plant cells?Disarmed retroviruses are used as vectors to deliver genes into which cells?The Ti plasmid was modified into a cloning vector from which organism?Bacterial cells are made competent to take up DNA by treating them with what?In the competent-cell method, bacteria get a brief heat shock at what temperature?In micro-injection, DNA is injected directly into what?In the gene gun method, cells are bombarded with DNA coated on what?Which enzyme is used to break open plant cells to release DNA?Which enzyme removes RNA while purifying DNA?Purified DNA precipitates out on adding which reagent?The DNA polymerase used in PCR is isolated from which bacterium?Repeated PCR cycles can amplify a DNA segment roughly how many times?What are the primers used in PCR?Which type of bioreactor is most commonly used?What does the stirrer do in a stirred-tank bioreactor?Separation and purification of a product after biosynthesis are together called what?In continuous culture, cells are kept in which growth phase?A protein made by a gene expressed in a heterologous host is called what?Which two core techniques gave birth to modern biotechnology?How does genetic engineering improve on traditional hybridisation?Which bacterium is the source of the Bt toxin gene used in crops?What activates the inactive Bt protoxin inside an insect?The cry genes cryIAc and cryIIAb control which pest in cotton?What is RNA interference (RNAi)?Which crop was attacked by the nematode Meloidegyne incognitia in the RNAi example?What is totipotency?Producing thousands of plants through tissue culture is called what?Plants produced by micro-propagation from one parent are known as what?Which plant part is cultured to obtain virus-free plants?Plants formed from fused protoplasts of two varieties are called what?Pomato was produced by fusing protoplasts of which two plants?What are plants, bacteria, fungi and animals with manipulated genes called?Golden rice is enriched with which nutrient?How are the two chains of insulin held together?Which part of pro-insulin is removed during maturation?Which company first prepared human insulin chains in E. coli plasmids in 1983?The first clinical gene therapy in 1990 treated a child with which deficiency?In ADA gene therapy, a functional ADA cDNA is introduced into cells using what?PCR is routinely used for early detection of which infection?ELISA works on which principle?Which animal makes up over 95 percent of transgenic animals?Which human protein from transgenic animals is used to treat emphysema?In 1997 a patent on which Indian crop was granted by the US Patent Office?What is biopiracy?Transgenic mice are being developed to test the safety of which vaccine?