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PUCET (Punjab) Biotechnology Applications — practice questions

Explore 2 free Biotechnology Applications questions in PUCET (Punjab) Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology Applications questions and exercises with answers

Showing questions 1–2 of 2.

To isolate DNA from bacterial cells, the cell wall is digested by:Read the answer and worked solution →After repeated PCR cycles, the gene of interest can be amplified to approximately:Read the answer and worked solution →

Worked practice questions

To isolate DNA from bacterial cells, the cell wall is digested by:

  1. Lysozyme
  2. Trypsin
  3. Chitinase
  4. Cellulase
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Correct answer: A. Lysozyme

**Cell-wall digesting enzymes**: **Lysozyme** for bacteria, **cellulase** for plants, **chitinase** for fungi. After cell lysis, **proteins → protease**; **RNA → ribonuclease**. Finally, DNA precipitates with **chilled ethanol** as a spool of fine threads.

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After repeated PCR cycles, the gene of interest can be amplified to approximately:

  1. ~100 copies
  2. ~10⁶ copies
  3. ~10²⁰ copies
  4. ~10⁹ copies (1 billion) in ~30 cycles
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Correct answer: D. ~10⁹ copies (1 billion) in ~30 cycles

Each cycle doubles the target → **2ⁿ copies after n cycles**. After ~30 cycles: ~2³⁰ ≈ **10⁹ (~1 billion) copies** — enough to clone, sequence, or use directly. Your syllabus explicitly states this.

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