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ISC Class 12 Biotechnology and Applications — practice questions — page 3

Explore 84 free Biotechnology and Applications questions in ISC Class 12 Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 61–84 of 84.

In gel electrophoresis, DNA fragments move toward the:Read the answer and worked solution →Separated DNA bands become visible after staining with:Read the answer and worked solution →Loss of gene function caused by a DNA insert inside it is:Read the answer and worked solution →Which plasmid was reworked into a cloning vector for plants?Read the answer and worked solution →Match each biotechnology term to its meaning.Read the answer and worked solution →True or false about transgenic workRead the answer and worked solution →Fill the gaps in the cloning storyRead the answer and worked solution →Restriction enzymes are popularly called:Read the answer and worked solution →Which enzyme is used to open plant cells before DNA isolation?Read the answer and worked solution →Purified DNA finally precipitates out on adding chilled:Read the answer and worked solution →Separation and purification of the final product is called:Read the answer and worked solution →The three sequential steps of one PCR cycle are:Read the answer and worked solution →Taq DNA polymerase used in PCR is obtained from:Read the answer and worked solution →Hind II cuts DNA on recognising a specific sequence of:Read the answer and worked solution →PCR is now used routinely to detect which virus?Read the answer and worked solution →Most transgenic animals produced so far have been:Read the answer and worked solution →Rosie, the first transgenic cow, gave milk enriched in human:Read the answer and worked solution →Transgenic mice are being used to test the safety of the:Read the answer and worked solution →Which body clears the safety of GM organisms in India?Read the answer and worked solution →The genes cryIAc and cryIIAb protect cotton against:Read the answer and worked solution →Bt cotton is resistant to BOLLWORM pests because it carries a gene from:Read the answer and worked solution →Which enzyme joins two cut DNA ends to make recombinant DNA?Read the answer and worked solution →The power of any plant cell to grow into a whole plant is:Read the answer and worked solution →In recombinant DNA technology, plasmids are commonly used as:Read the answer and worked solution →

Worked practice questions

In gel electrophoresis, DNA fragments move toward the:

  1. Negative electrode (cathode), as DNA carries positive charge
  2. Positive electrode (anode), because DNA is negatively charged
  3. Neither electrode, since DNA is neutral
  4. Both electrodes equally, the field randomises movement
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Correct answer: B. Positive electrode (anode), because DNA is negatively charged

DNA has a negatively-charged phosphate backbone, so it moves toward the POSITIVE electrode (anode). Smaller fragments migrate faster through the gel pores. EtBr or SYBR Green stains DNA for UV visualisation.

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Separated DNA bands become visible after staining with:

  1. Ammonium chloride
  2. Magnesium sulphate
  3. Ethidium bromide
  4. Potassium bromide
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Correct answer: C. Ethidium bromide

1. Pure DNA cannot be seen in visible light. 2. Ethidium bromide binds the DNA in the gel. 3. Under ultraviolet light the bands glow orange. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Loss of gene function caused by a DNA insert inside it is:

  1. Insertional inactivation
  2. Selectable marking
  3. Palindromic cutting
  4. Downstream processing
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Correct answer: A. Insertional inactivation

1. The insert lands in the middle of a coding sequence. 2. The reading of that gene is broken. 3. This is termed insertional inactivation. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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More Biology topics

Genetics and EvolutionReproduction in Organisms & PlantsBiology in Human Welfare & HealthEcology and Environment