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CUET UG Biotechnology and Applications — practice questions — page 3

Explore 112 free Biotechnology and Applications questions in CUET UG Biology. Open each question or exercise to read its content, reference answers and available explanation.

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Biotechnology and Applications questions and exercises with answers

Showing questions 61–90 of 112.

In gel electrophoresis DNA fragments move towards the:Read the answer and worked solution →Which matrix is most commonly used to separate DNA fragments?Read the answer and worked solution →In an agarose gel the fragment that travels farthest is the:Read the answer and worked solution →PCR amplifies a chosen segment using two sets of:Read the answer and worked solution →Repeated cycles of PCR can amplify a segment about:Read the answer and worked solution →The vector sequence from which replication begins is the:Read the answer and worked solution →An antibiotic resistance gene on a vector serves mainly as a:Read the answer and worked solution →A foreign gene ligated at the BamH I site of pBR322 destroys:Read the answer and worked solution →The thermostable polymerase used in PCR is taken from:Read the answer and worked solution →Virus free plants can be raised from an infected plant using its:Read the answer and worked solution →The Bt toxin used in pest resistant crops comes from a:Read the answer and worked solution →Silencing a gene with a complementary double stranded RNA is:Read the answer and worked solution →Raising thousands of plants through tissue culture is called:Read the answer and worked solution →Plants raised from one parent by tissue culture are called:Read the answer and worked solution →Plant cells stripped of their walls by digestion are called:Read the answer and worked solution →Fusing protoplasts of two varieties gives a plant known as a:Read the answer and worked solution →Rice enriched with vitamin A by genetic modification is:Read the answer and worked solution →Inside Bacillus thuringiensis the Bt toxin is stored as:Read the answer and worked solution →The Bt protoxin becomes an active toxin because the insect gut is:Read the answer and worked solution →Once activated the Bt toxin kills by acting on the insect:Read the answer and worked solution →Tobacco roots are protected from a nematode by silencing its:Read the answer and worked solution →ELISA works on the principle of interaction between:Read the answer and worked solution →In molecular diagnosis the labelled single strand used is called a:Read the answer and worked solution →Using a country's bio-resources without authorisation is:Read the answer and worked solution →How many documented varieties of Basmati are grown in India?Read the answer and worked solution →The ADA gene is carried into a patient's lymphocytes by a:Read the answer and worked solution →Very low numbers of a pathogen can be detected early by:Read the answer and worked solution →Recombinant DNA uptake is helped by a brief heat shock at:Read the answer and worked solution →The first restriction endonuclease to be isolated was:Read the answer and worked solution →Cutting a stained DNA band out of the gel and recovering it is:Read the answer and worked solution →

Worked practice questions

In gel electrophoresis DNA fragments move towards the:

  1. Cathode
  2. Buffer
  3. Matrix
  4. Anode
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Correct answer: D. Anode

1. DNA carries a negative charge on its backbone. 2. A negative molecule is pulled to the positive electrode. 3. That electrode is the anode. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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Which matrix is most commonly used to separate DNA fragments?

  1. Agarose
  2. Gelatin
  3. Alumina
  4. Amylose
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Correct answer: A. Agarose

1. The matrix must sieve fragments by size. 2. Agarose is a natural polymer from sea weeds. 3. It is the matrix in common use today. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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In an agarose gel the fragment that travels farthest is the:

  1. Longest one
  2. Smallest one
  3. Largest one
  4. Heaviest one
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Correct answer: B. Smallest one

1. The gel acts as a sieve. 2. Small fragments slip through the mesh easily. 3. So the smaller the fragment, the farther it moves. _Source: syllabus Biology, Ch 9 'Biotechnology : Principles and Processes'_

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