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The phosphodiester bond of the DNA backbone joins which two carbons?
AThe 3' and the 1' carbons
BThe 5' and the 3' carbons
CThe 1' and the 2' carbons
DThe 2' and the 4' carbons
Answer & Solution
Correct answer: B. The 5' and the 3' carbons
1. The backbone alternates sugar and phosphate along the whole strand.
2. The phosphate residue is attached to the hydroxyl group of the 5 prime carbon of one sugar of one nucleotide.
3. It is also attached to the hydroxyl group of the 3 prime carbon of the sugar of the next nucleotide.
4. That pairing forms a 5 prime to 3 prime phosphodiester bond.
5. The 1 prime carbon carries the base instead, so it is not part of the backbone linkage.
_Source: OpenStax Biology for AP(R) Courses (CC BY 4.0), Ch 14 'DNA Structure and Function', sections 14.1-14.6_
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