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In eukaryotic transcription, RNA polymerase cannot start the process alone. What must happen first for transcription to begin?

ARNA polymerase must bind the operator sequence used in prokaryotic operons
BTranscription factors must bind the promoter region first and recruit RNA polymerase
CRibosomes must first assemble on the DNA before RNA polymerase arrives
DHistones must be permanently removed from the entire chromosome
Answer & Solution
Correct answer: B. Transcription factors must bind the promoter region first and recruit RNA polymerase
1. Unlike in prokaryotic cells, eukaryotic RNA polymerase requires other proteins called transcription factors to begin transcription. 2. RNA polymerase by itself cannot initiate transcription in eukaryotic cells. 3. Transcription factors must bind the promoter region first and recruit RNA polymerase to the site. 4. Operators are a prokaryotic structure, and ribosomes act during translation, not during the initiation of transcription. _Source: OpenStax Biology (1st ed., CC BY 4.0), Ch 16 "Gene Expression", section 16.4 Eukaryotic Transcription Gene Regulation_
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