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During DNA replication, the short DNA segments synthesised on the lagging strand are joined together by which enzyme?
AHelicase
BDNA ligase
CTopoisomerase
DDNA polymerase
Answer & Solution
Correct answer: B. DNA ligase
1. On the lagging strand, DNA is made in short pieces called Okazaki fragments.
2. These fragments must be joined into a continuous strand.
3. The enzyme that joins them is DNA ligase.
4. Helicase unwinds the strands and DNA polymerase adds nucleotides, but neither joins the fragments, which rules out options A and D.
5. So the joining enzyme is DNA ligase, option B.
_Source: Samacheer Kalvi (TN SCERT) Class 10 Science, Unit 18 Genetics "DNA Replication", p.275_
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