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Replication does not initiate randomly along a DNA molecule; it starts at:
AThe 3′ end always
BThe 5′ end always
CAnywhere in the molecule
DSpecific sites called origins of replication (ori)
Answer & Solution
Correct answer: D. Specific sites called origins of replication (ori)
Replication initiates at **origins of replication (ori)** — defined DNA sequences recognised by initiator proteins. This is why plasmids/vectors used in recombinant DNA technology must contain an origin to replicate inside a host cell.
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